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Adherence assays and Slime production of Vibrio alginolyticus and Vibrio parahaemolyticus BJM
Abdallah,Fethi Ben; Chaieb,Kamel; Zmantar,Tarek; Kallel,Hela; Bakhrouf,Amina.
In this study we investigated the phenotypic slime production of Vibrio alginolyticus and Vibrio parahaemolyticus strains, food-borne pathogens, using a Congo red agar plate assay. Furthermore, we studied their ability to adhere to abiotic surfaces and Vero cells line. Our results showed that only V. alginolyticus ATCC 17749 was a slime-producer developing almost black colonies on Congo red agar plate. Adherence to glace tube showed that all V. alginolyticus strains were more adherent than V. parahaemolyticus. Only V. alginolyticus ATCC 17749 was found to be able to form biofilm on polystyrene microplate wells (OD570 = 0.532). Adherence to Vero cells showed that all tested strains were non adherent after 30 min, however after 60 min all the studied strains...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Vibrio; Slime production; Adherence; Abiotic surface; Vero cells.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200033
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Modulation of drug resistance and biofilm formation of Staphylococcus aureus isolated from the oral cavity of Tunisian children BJID
Zmantar,Tarek; Ben Slama,Rihab; Fdhila,Kais; Kouidhi,Bochra; Bakhrouf,Amina; Chaieb,Kamel.
Abstract Objectives: This study aims to investigate the antimicrobial and the anti-biofilm activities of Lactobacillus plantarum extract (LPE) against a panel of oral Staphylococcus aureus (n = 9) and S. aureus ATCC 25923. The in vitro ability of LPE to modulate bacterial resistance to tetracycline, benzalchonium chloride, and chlorhexidine were tested also. Methods: The minimum inhibitory concentrations (MICs) and the minimal bactericidal concentrations of Lactobacillus plantarum extract, tetracycline, benzalchonium chloride and clohrhexidine were determined in absence and in presence of a sub-MIC doses of LPE (1/2 MIC). In addition, the LPE potential to inhibit biofilm formation was assessed by microtiter plate and atomic force microscopy assays....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antibacterial; Staphylococcus aureus; Biofilm; Antibiotics; Synergetic; Lactobacillus.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000100027
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